Cyanobacteria Spirulina Platensis Basic Protein C-Phycocyanin and Zn(II) Ions
نویسنده
چکیده
The interaction of Zn(II) ions with cyanobacteria Spirulina platensis basic protein C-phycocyanin (C-PC) is studied by fluorescence spectroscopy.Stern–Volmer quenching constant value for Zn(II)–C-PC is determined. The binding energy of Zn(II) ions with C-phycocyanin is determined using equilibrium dialysis and atomic absorption spectroscopy. Cooperative binding of Zn(II) ions with C-phycocyanin is observed. The binding constants diminished with increasing ionic strength, suggesting an adaptive protective response. “Nonelectrostatic” and polyelectrolyte components of binding free energy for Ag + , Cu 2+ , Cr 3+ , Pb 2+ , Ni 2+ , and Zn 2+ –C-phycocyanin (Spirulina platensis) complexes are determined. It is shown that “nonelectrostatic” component of binding free energy is dominating at the metal–C-PC interaction, while the polyelectrolyte contribution being less important, and the “nonelectrostatic” forces contribution for Ag + –C-phycocyanin (Spirulina platensis) complexes exceeds that for other metal ions.
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